Just read a fresh paper from Edward Lemke group in EMBL in last issue of Angewandte Chemie. They have 'adjusted' the SPIEDAC (Strain promoted Inverse Electron Demand Diels–Alder cycloaddition) such that now thay can efficiently dual label proteins in cell that bear byclononyne (BCN) and transcyclocotyne with a mixture of tetrazine and methyl-derivative of tetrazine containing dyes.
It was well known that methyl-derivatized tetrazines are more stable and less reactive. I did not expect though that it will be so much less reactive so it will not be coupling with transcyclooctyne (see the picture). Great job!
No comments:
Post a Comment